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Abstract
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Phylogenetic analysis of 16S-rDNA sequences of isolated bacteria from hemolymph of infected Galleria mellonella cadavers with entomopathogenic nematodes revealed different strains of non-symbiont bacteria. Biochemical tests and phylogenetic analysis using Maximum Parsimony, Maximum Likelihood and Neighbor Joining methods were done and two species including three strain of Providencia rettgeri and four strains of Pseudochrobactrum sp. were identified to be associated with H. bacteriophora and S. carpocapsae, respectively. As a supplementary tool, RNA secondary structure and minimum free energy was involved in analysis for more confirmation
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