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Keywords
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stanniocalcin-1, NF-ƙB subunit p65, expression level,
colorectal cancer, clinicopathological features
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Abstract
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Background: Stanniocalcin-1 (STC1) and nuclear factor (NF)-ƙB subunit p65 transcription factor are involved in
various types of human malignancies. The roles of STC1 and NFƙB-p65 in colorectal cancer (CRC) are still not
fully understood. We investigated expression levels of NF-ƙB p65 and STC1 and also correlations between STC1
and NF-ƙB p65 expression and clinicopathological features in CRC.
Methods: Tumor tissue samples were collected from 48 patients with CRC. RT-PCR and Real-time PCR analysis
was performed to examine mRNA levels of STC1 and NF-ƙB p65.
Results: The relative mRNA levels of STC1 and NF-ƙB p65 were significantly higher in tumor tissues than in adjacent
mucosa (p = 0.025 and p = 0.044, respectively). The data also showed that STC1 and NF-ƙB p65 mRNA levels
were not significantly associated with clinicopathological characteristics. In addition, there was no association between
expression levels of STC1 and NF-ƙB p65 in tumor samples.
Conclusions: Our data indicate that STC1 and NF-κBp65 is activated constitutively in colorectal carcinoma tissues,
suggesting that activation of these factors might play an important role in colorectal tumorigenesis. Future
studies should examine STC1 and NF-κBp65 as a molecular target for the treatment of CRC.
(Clin. Lab. 2016;62:xx-xx. DOI: 10.7754/Clin.Lab.2015.150827)
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