|
Abstract
|
The brine shrimp, Artemia is the dominant macrozooplankton present in many hypersaline envi-
ronments. Artemia urmiana is the only macroscopic organism in Urmia Salt Lake (Iran), and the high salinity
of the lake makes it a suitable environment for halophilic archaea too. Because of common environment for
Artemia and extreme halophiles; this investigation is concentrated on studying the relationship between Arte-
mia and halophilic archaea in Urmia Lake. In this study first the procedure of arhaea isolation was done.
Then, isolated strains were sub-cultured and DNA was extracted and amplified by PCR using specific prim-
ers for amplifying archaeal 16S rRNA. The amplified archeal DNA fragments were purified, and sequenced.
16S rRNA sequences were compared to known sequences using the NCBI BLAST program. Sequences relat-
ing to Halorubrum, Haloarcula and Halobacterium species were identified in Urmia Salt Lake water and Arte-
mia adults and the phylogenetic tree of different species was constructed. Only Halorubrum species were pres-
ent in association with Artemia. They belong to Halobacteriaceae family of archeae which are isolated from
different salt lakes in different parts of world and we could show their existence in adult Artemia, another
organism living in hypersaline enviroments
|