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Abstract
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A new method based on the combination of ultrasound-assisted magnetic solid phase extraction (USAMSPE)
and high performance liquid chromatography-UV-Vis (HPLC-UV-Vis) was developed for the
isolation and preconcentration of atorvastatin (AT) from urine, plasma and water samples using sodium
dodecyl sulfate coated Fe3O4 magnetic nanoparticles (MNPs) as a sorbent. The properties of Fe3O4 NPs
including strong magnetism and high surface area were utilized effectively in the MSPE process. The
prepared magnetic nanoparticles were characterized by transmission electron microscopy and X-ray
diffraction. The effects of different parameters influencing the extraction efficiency including pH, the
amount of Fe3O4 nanoparticles, the amount of SDS, extraction time, desorption solvent, desorption time
and solvent volume were optimized. Additionally, the effect of sample volume on the extraction recovery
of AT was studied. Under optimized conditions, the method was successfully applied to the extraction of
atorvastatin from water samples and an absolute recovery of 99.8%, a detection limit of 0.1 ng L1 and
a relative standard deviation of <6.8% were obtained. The method linear dynamic range is in the range of
0.4–500 mg L1 with R2 ¼ 0.9999. The relative recoveries in different aquatic and biological matrices
were investigated and values of 88.9 to 109.5% were obtained.
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