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Abstract
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tIn this work, the magnetic molecularly imprinted polymer nanoparticles (MMIP-NPs) for the selectivepre-concentration of Tizanidine have been described. The polymer nanoparticles were synthesized bythe polymerization of methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as across-linker, 2,2-azobisisobutyronitrile as an initiator and Tizanidine as a template molecule. The MMIP-NPs were characterized by scanning electron microscopy (SEM) and thermogravimeteric analysis (TGA).Imprinted Tizanidine molecules were removed from the polymeric structure using acetic acid in methanol(10:90 V/V%), as the eluent solvent. The limits of detection (L.O.D) for Tizanidine were 1.13 × 10−6M and1.68 × 10−6M in ultrapure water and urine, respectively. Also, the relative standard deviations (R.S.D) inultrapure water and urine were 2.21% and 2.58%, respectively. The method was applied to the determi-nation of Tizanidine in the human urine samples.
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