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Abstract
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In order to investigate cold tolerance in 20 barley genotypes based
on crown survival percentage, a greenhouse experiment was
conducted as split plots with three replicates, with temperatures
(-8, -10, -12, -14 and -16°C) as main plots and barley genotypes
constituting subplots. Randomized complete block design
was performed to analyze physiological traits measured after
acclimation and before applying chilling temperatures. Crown
survival percentage was measured zero at -16°C. Error was not
significant for main factor in split plots, therefore, data analysis for
-8, -10, -12 and -14°C was executed as factorial. Results indicated
that temperature, genotype and their interactions had significant
influence on the crown survival percentage. Also, the genotypes
were significantly different in terms of LT50, the glycine betaine
content and leaf relative water content before and after adaptation
to cold. Comparison of the means, based on LT50 and crown
survival percentage, suggested the genotype number 15 (with
K-096M3 pedigree) as the most tolerant to crown freezing, and
genotypes 36 (Schulyer), 15 (K-096M3) and 14 (GK Omega) as
possessing the most desirable physiological traits, with genotypes
15 and 36 possessed the lowest difference before and after leaf
relative water content, and the maximum quantity of glycine betaine
after adaptation to cold. Cluster analysis of the genotypes, based on
the aforementioned traits, divided them into three distinct tolerant,
semi- tolerant and sensitive groups.
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