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Abstract
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ABSTRACT: Capparis spinosa L. (Caper) is a multipurpose crop that can be used for ornamental, culinary,
cosmetics, pharmaceutical and medicinal purposes. As ornamental shrub, caper plant is used for the
prevention of soil erosion and help to conserve soil water reserves. Every part of caper has medicinal uses.
Due to the great population diversity and difficulties in conventional propagation methods, for the protection
of C. spinosa from genetic erosion; a tissue culture method would be a good way for mass propagation of this
plant. In vitro propagation of caper was begun with seeds. The highest shoots proliferation was obtained on
MS medium supplemented with 3 mgl-1 cytokinin. Varzegan cultivar had the highest shoot and nod number
and shoot length. The results showed that Zeatin was quite effective in the proliferation of capper explants.
More Shoot multiplication was obtained at the same medium by sub-culturing shoot segment with 2-3 nodes
every seven weeks (for two times). Auxin type (IBA and NAA), levels (0, 1 and 2 mgl-1) and media (½MS, MS)
as well as cultivar (Varzegan and Miyaneh) had profound effects on root number. The result showed that the
highest root number was obtained from Varzegan cultivar. With increasing auxins concentration rooting
percentage was increased. The greatest rooting response shoots was acquired from MS media supplemented
with 2 mgL-1 of NAA.
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