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Keywords
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biomaterials, ceramics, ceramic matrix, magnetic
materials, nanomaterials
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Abstract
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Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material
that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and
bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic
scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The
present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One
scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic
material (Fe3O4 nanoparticles). Disk-shaped (10 mm32 mm) samples of the two scaffolds were prepared.
Primary human fibroblast proliferation was 1.8- and 2.5-fold faster, respectively, when cultured in the
presence of hydroxyapatite or ferrous nanoparticle/hydroxyapatite mixtures. Optical microscopy images
revealed that the increased proliferation was due to enhanced cell-cell contact. The presence of magnetic
Fe3O4 nanoparticles in the ceramic scaffolds significantly increased cell proliferation compared to
hydroxyapatite scaffolds and tissue culture polystyrene.
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