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Abstract
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In this study a new version of solid phase extraction performed in a narrow–bore tube using large volumeof sample
followed by dispersive liquid–liquid microextraction has been proposed to achieve a high sensitive analytical
method. It has been used for the extraction and preconcentration of trace concentrations of some pesticides
(metalaxyl, haloxyfop–r–methyl, clodinafop–propargyl, diclofop–methyl, and bromopropylate) in different vegetables
(potato, tomato, onion, garlic, celery, radish, beet, and carrot) followed by gas chromatography–flame
ionization detection. In the proposed method mg–level of a sorbent (C8) is mixed with a dispersive solvent
(iso–propanol) and then dispersed into an aqueous solution placed into a narrow–bore tube. By this action,
the sorbent passes through the solution in a short time instead of passing the solution from the cartridge in conventional
solid phase extraction in a long time. To achieve high enrichment factors, an appropriate organic solvent
(acetonitrile) is used to elute the compounds of interest from the sorbent for the following dispersive
liquid–liquid microextraction procedure. Some important parameters which affect the extraction efficiency
were studied. Under the optimum extraction conditions, the method showed wide linear ranges (0.08–
10,000 μg L−1). Enrichment factors and extraction recoveries were in the ranges of 1702–4147 and 31–75%, respectively.
Detection limits were in the range of 0.02–0.25 μg L−1 in solution (0.01–0.89 μg kg−1 in samples).
LOQs were obtained in the range of 0.08–0.83 μg L−1 in solution (0.04–2.9 μg kg−1 in samples). Relative standard
deviations of 4–7% (n=6) and 2–4% (n = 7) were obtained for 10 and 50 μg L−1 of each analyte, respectively.
The relative recoveries obtained in three different concentrations were 80–101%.
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