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Abstract
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Graphene, a new class of 2D carbon nanomaterials with one atom thickness, has attracted
considerable attention in recent years [1]. Eggshell membrane is a cheap, green and easily
achievable biopolymer which has been used frequently in analytical science [2]. Malachite
green (MG) is a triphenylmethane dye, originally used as a dyeing agent in the textile
industry, has also been widely used in the fish farming industry during the last decades. The
reason for its popularity is derived from its broad antimicrobial spectrum and effectiveness in
the prevention and treatment of certain fish diseases compared to other fishery chemicals.
In present study, graphene oxide doped soluble eggshell membrane nanocomposite
(GO/SEP) was doped with magnetic nanoparticles via a simple co-precipitation method. The
resulted GO/SEP/Fe3O4 nanocomposite was used as an efficient nano-sorbent for
preconcentration and determination of Malachite green using spectrophotometric method. The
resulted method could be used effectively for the determination of Malachite green in the
range 0.5-250 ng mL-1 with detection limit of 0.2 ng mL-1. Preconcentration factor and
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