|
Keywords
|
magnetic nano-sorbent, solid-phase extraction, atenolol, human serum, pharmaceutical wastewater samples
|
|
Abstract
|
Styrene-maleic anhydride (SMA) copolymer is a commercial industrial polymer which is
cheaper than any other polymers possessing reactive group in the main chain or side chain for
further functionalization [1, 2]. In this work, Fe3O4 magnetic nano-particles were firstly
synthesized and their surfaces were modified with 3-aminopropyltriethoxysilane (APTES).
Then, styrene-maleic anhydride copolymer was synthesized by free radical polymerization of
styrene and maleic anhydride, and after grafting with 3-aminbenzoic acid, the modified
copolymer was reacted with amine terminated Fe3O4 NPs to prepare magnetic nanocomposite
(MNC). After characterization of the synthesized MNC by Fourier transform
infrared spectroscopy, field emission scanning electron microscopy and X-ray diffraction, the
obtained material was applied as a novel nano-sorbent for selective extraction and preconcentration
of atenolol prior to its determination by spectrofluorometry. This prepared
nano-hybrid possesses the magnetism property of Fe3O4 nano-particles that makes it easily
manipulated by an external magnetic field. On the other hand, the surface modification of
SMA by APTES leads to selective separation of the target analyte from sample matrices. The
experimental factors affecting the extraction/pre-concentration and determination of the
analyte were investigated and optimized. Under the optimized experimental conditions, the
calibration graph was linear in the range between 0.10 and 60 ng mL−1, with a correlation
coefficient of 0.9989. The limits of detection and enrichment factor for atenolol were 0.32 ng
mL−1 and 100 ng mL−1, respectively. The maximum sorption capacity of the adsorbent for
atenolol was 96.7 mg g−1. The method was successfully applied to monitoring atenolol in
human serum and pharmaceutical wastewater samples, with recoveries in the range of 96.0%-
103.0% for the spiked samples.
|