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Abstract
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Here, Ag nanoparticle/flake-like Zn-based MOF nanocomposite (AgNPs@ZnMOF) with great peroxidase-like
activity was applied as an efficient support for molecularly imprinted polymer (MIP) and successfully used for
selective determination of patulin. AgNPs@ZnMOF was simply synthesized by creating Ag nanoparticles (Ag
NPs) inside the nano-pores of flake-like (Zn)MOF. The high surface area of MOF remarkably improved the
catalytic activity of Ag NPs which was assessed by fluorometric, colorimetric and electrochemical techniques.
Furthermore, it was observed that patulin could strangely reduce the catalytic activity of AgNPs@ZnMOF,
probably due to its electron capturing features. This outcome was the motivation to design an assay for patulin
detection. In order to make a selective interaction with patulin molecules, MIP layer was created on the surface
of AgNPs@ZnMOF by co-polymerization reaction of 3-aminopropyl triethoxysilane (APTES) and tetraethyl orthosilicate
(TEOS) monomers wherein patulin was applied as template agent. Combination between the selective
identifying feature of MIP and outstanding peroxidase-like activity of novel AgNPs@ZnMOF nanocomposite as
well as the sensitive fluorescence detection system was led to the design of a reliable probe for patulin. The
prepared MIP-capped AgNPs@ZnMOF catalyzed the H2O2-terephthalic acid reaction which produced a high
florescent product. In the presence of patulin, the fluorescence intensity was decreased proportional to its
concentration in the range of 0.1–10 μmol L−1 with a detection limit of 0.06 μmol L−1. The proposed method
was able to selectively measure patulin in a complex media without significant interfering effects from analogue
compounds.
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