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Abstract
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Abstract
Background: Many case-control investigations have showed the correlation of TP53 gene polymorphisms with the risk of breast cancer. However, the findings were not consistent. It has been suggested that the investigation of P53 genotype combinations and haplotypes may be more helpful than single polymorphisms.Inthe present study, we investigated the association of P53 intron 3 and codon 72 polymorphisms, as well as their Haplotypes and genotype combinations,with the development of breast cancer among Azeri women of Iran.
Methods:A total of 143 Iranian-Azeri females suffering from breast cancer and 160ethnically and age-matched healthy females were participated in this study. Intron 3 genotype was indicatedbylength analysis of PCR ampliconon polyacrylamide gels andallele specific–polymerase chain reaction(AS-PCR) wasapplied for genotyping Arg72Pro variation. Data analysis was performed usingthe javastat online statistics package and SHEsis online program.
Results:Our findings did not show a significant association between P53 intron 3 and codon 72 polymorphisms and the risk of breast neoplastic tumors among Iranian-Azeri women. However, the (-16ins/+16ins) (Arg/Arg) combined genotype and (+16Ins-Arg) haplotype had a higher frequency in patients in comparison with control group(OR=3.816; 95%CI: 0.906-18.459; P=0.047 and OR=3.941; 95%CI: 1.583-9.812; P=0.002, respectively).
Conclusion: In our study, there weresignificant correlations between (-16ins/+16ins) (Arg/Arg) genotype combination and(+16ins-Arg) haplotype with the increasedsusceptibility tobreast cancer development in Iranian-Azeri females.
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