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Abstract
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A novel moderately halophilic, Gramstain
negative and aerobic bacterium, designated
strain TBZ21T, was isolated from a water sample of
Urmia Lake, Iran. Cells were observed to be nonmotile
rods with no flagellum, showing positive
catalase and oxidase reactions. Strain TBZ21T was
found to grow at 10–40 C (optimum, 30 C), at pH
7–10 (optimum, pH 8) and in the presence of 1–22%
(optimum, 10%). The major fatty acids were identified
as C19:0 cyclo x8c, C16:0, Summed features 3 (C13:0
3-OH and/or iso-C15:1 H) and 8 (C18:1 x7c and/or C18:1
x6c) and C12:0 3-OH. The major polar lipids were
identified as diphosphatidylglycerol, phosphatidylglycerol,
phosphatidylethanolamine and an unidentified
phosphoaminolipid. The genomic DNA G?C
content of strain TBZ21T was determined to be
63.3 mol%. Phylogenetic analyses based on 16S
rRNA gene sequences indicated that strain TBZ21T
belongs to the genus Halomonas, and shows high
sequence similarities to Halomonas fontilapidosi
5CRT (99.43%), Halomonas ventosae AL12T
(98.64%), Halomonas sediminicola CPS11T
(98.63%) and Halomonas aestuarii Hb3T (98.08%),
and has low similarities (below 98.0%) with other
members of the genus. The values of DNA–DNA
relatedness between strain TBZ21T and the closely
related strains H. fontilapidosi LMG 24455T and H.ventosae LMG 26187T were 42 ± 11% and
54 ± 16%, respectively. On the basis of phenotypic,
chemotaxonomic and molecular characteristics, strain
TBZ21T is concluded to represent a novel species of
the genus Halomonas, for which the name Halomonas
tabrizica sp. nov. is proposed. The type strain is
TBZ21T (=LMG 25445T=DSM 23018T).
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