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Abstract
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Chemiluminescence (CL), as an analytical technique, is based on the measurement of the electromagnetical radiation (usually visible/near-infrared radiation) emitted by a chemical reaction [1]. In recent years, nanomaterials with the unique physical and chemical properties have amplified the CL signal and improved the sensitivity of the various CL detection systems [2]. In this work, graphene oxide (GO) nanoparticles (NPs) was synthesized by using the modified Hummer's method [3]. The structural study of GO was performed by ultraviolet and visible (UV-VIS) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. It was found that GO NPs possess oxidase-like activity and enhance the intensity of the luminol–O2 CL reaction. Moreover, in the presence of paracetamol as a target analyte, the improved GO NPs-luminol-O2 CL intensity was decreased. Accordingly, a novel and sensitive CL method for determination of paracetamol drug was developed. The effect of various chemical parameters on CL intensity were investigated and optimized. Under the optimum conditions, a linear relationship was obtained between the enhanced CL intensity and the concentration of paracetamol in the range of 7×10‒6 ‒5×10‒5 M with a detection limit of 8.72×10-7M. The relative standard deviation for 5 determinations of 2×10‒5M paracetamol is 3.39%. The method was applied to determine paracetamol in several pharmaceutical formulations.
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