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Abstract
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A novel magnetic solid-phase extraction technique based on a ternary nano-composite, magnetite/reduced graphene oxide/silver, as a nano-sorbent was developed for simultaneous extraction/preconcentration and measurement of morphine and codeine in biological samples by high-performance liquid chromatography. The magnetic ternary nano-composite was synthesized and its functional groups, morphological structure, and magnetic properties were characterized by field emission scanning electron microscopy, vibrating sample magnetometer, powder X-ray diffraction, energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy. The optimizing of the significant variables affecting the extraction process was evaluated by a response surface methodology. In the optimized conditions, the constructed calibration curves for morphine and codeine are linear in the range of 0.01‒10 μg L−1 with correlation coefficients of 0.9983 and 0.9976, respectively. The detection limit and enrichment factor for morphine and codeine are 1.8 ng L−1, 1000 and 2.1 ng L−1, 1000, respectively. The presented technique was employed for the monitoring of morphine and codeine in numerous blood and urine samples with relative recoveries between 97.0 and 102.5%, and relative standard deviations of 1.02–5.10% for the spiked samples.
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