|
Abstract
|
In this study, a sensitive nicotinamide adenine
dinucleotide (NADH) biosensor based on Au-Copper
oxide nanocomposite modified carbon ceramic electrode
(Au CuO/CCE) was introduced. The developed NADH
biosensor was prepared by controlled electrodeposition of
copper and Au nanoparticles on the surface of a renewable
CCE and was turned to Au CuO/CCE by cycling
the potential in alkaline media. The prepared electrode
was carefully characterized with scanning electron microscopy,
X-ray diffraction, atomic force microscopy and
cyclic voltammetry techniques. According to scan rate
study, surface coverage (Γ) of the fabricated Au CuO/
CCE was calculated to be 1.54×10 8 molcm 2 which was 3
time more than CuO/CCE. The fabricated electrode is
well stable which could be reliably utilized for the
determination of NADH with amperometry technique
over the concentration range of 1–29 μM with sensitivity
and detection limit (S/N=3) of 0.1025 μAμM 1 and
0.09 μM respectively. The prepared biosensor was used
for NADH determination in serum samples with fast
response time and satisfactory analytical results.
|