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Abstract
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A deep eutectic solvent-based ultrasound-assisted liquid-liquid microextraction (DES-UALLME) followed by
quantitative analysis using HPLC-UV has been developed for the determination of organophosphorus pesticides
(phosalone and chlorpyrifos) in fruit juice samples (red grape juice and sour cherry juice). In DES-UALLME,
ultrasonication was applied to transfer the aggregated of DES into tiny droplets. The parameters that influence
on extraction efficiency were studied and optimized by central composite design (CCD) in combination with a
desirability function approach. The optimum conditions obtained by multivariate optimization were 408 μL of
DES, 560 μL of THF, pH of 5.92, and an ultrasonication time of 12.31 min. Under the optimized condition, wide
linear range (1–500 ng mL−1), low detection limits (0.070 and 0.096 ng mL−1), high enrichment factors, and
good extraction recoveries were obtained. The procedure was successfully applied for the determination of
phosalone and chlorpyrifos in red grape juice and sour cherry juice samples with satisfactory relative recoveries
(87.3–116.7 %).
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