|
Abstract
|
Due to the increase of microbial resistance to antibiotics and the occurrence of side effects,
use of medicinal plants with anti-microbial properties seems to be rational. Hence, in this
study, some plants of the Apiaceae, Asteraceae, Brassicaceae, and Cucurbitaceae families
were evaluated for antimicrobial effects. The aerial parts of the plants were extracted by
different solvents using a Soxhlet apparatus. Subsequently, the inhibitory effect of the extracts
on different microbial species was assessed. Extracts with high growth inhibitory effect
were fractionated and their MIC was determined. Furthermore, primary phytochemical and
GC-MS analysis were used to identify the chemical compounds of potent samples of n-hexane
extracts of Eryngium caerulum (E. caeruleum) and Eryngium thyrsoideum (E. thyrsoideum.)
Both plants showed considerable antimicrobial activities against Staphylococcus epidermidis
among the fractions, 40% and 60% VLC fractions of n-hex extract of E. caeruleum and 40%
VLC fraction of n-hexane extract of E. thyrsoideum illustrated the most growth inhibitory
effect. Moreover, the results of preliminary phytochemical and GC-MS analysis confirmed
that steroids, fatty acids and terpenoids play an important role to show anti-microbial activity,
respectively. Among all samples, the 40% VLC fraction of n-hexane extract of E. thyrsoideum
for possessing high amounts of fatty acids and terpenoids indicated the most anti-microbial
potency.
|