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Abstract
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Immobilization of enzyme onto nanoparticles such as chitosan can have biotechnological
importance. In this study, chitosan nanoparticles (ChNPs) were prepared by
Ionic gelation method and Endoglucanase Cel9A from Alicyclobacillus acidocaldariius
(AaCel9A) immobilized on the nanoparticles. The FTIR results showed that the
enzymes were immobilized on the ChNPs. The dynamic light scattering and scanning
electron microscope (SEM) results illustrated that the AaCel9A-ChNPs approximately
had 40 nm diameters. For optimizing enzyme immobilization, response surface methodology
was employed using different variables (pH, enzyme immobilization time,
and enzyme to ChNPs ratio [E/Cs]). The results showed that the high immobilization
efficiency was achieved in pH 7, E/Cs of 0.4 in 2.63 hr. The enzyme activity results
showed that, immobilization increased optimum pH for activity (from 6.5 to 7.5) and
the enzyme Km (from 3.703 to 12.195 [mg/ml]), which make it suitable to use in some
industries such as detergents.
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