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Abstract
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Biocompatible, conductive, and hydrophilic 3D fibroblast scaffolds were prepared by using Poly(2-hydroxy
ethyl methacrylate)-co-poly(N-isopropylacrylamide)-co-poly(ε-caprolactone) (PHEMA-co-PNIPAAm-co-PCL)
terpolymers and polyaniline (PANI) nanocomposites. In terpolymer, PHEMA and PNIPAAm segments reflected
hydrophilicity and biocompatibility, and PCL led to appropriate mechanical characteristics. Blending of PANI and
terpolymer chains led to induction of conductivity to the terpolymer bulk. The morphology, electrical conductivity,
biocompatibility, mechanical properties and hydrophilicity of constructed nanofibers were investigated. To this end,
the cyclic voltammetry (CV) measurement, field emission scanning electron microscopic (FESEM) images, the contact
angle measurement, and the tensile test were conducted. The obtained scaffolds showed natural microenvironment of
extra cellular matrix (ECM) to regulate cell attachment, proliferation, and differentiation for electrical conductivity
and hydrophilicity. In vitro cytocompatibility investigations represented that nanofibers were non-toxic to mouse
fibroblast cells.
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