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Keywords
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biomedical analysis, bioreceptor, biotechnology, heme protein imprinting, polymer
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Abstract
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In this study, a selective and sensitive molecular imprinting-based electrochemical
sensors, for horseradish peroxidase (HRP) entrapment was fabricated using electro
polymerization of ß-Cyclodextrin (ß-CD) on the surface of glassy carbon electrode.
Poly beta-cyclodextrin P(ß-CD) provide efficient surface area for self-immobilization
of HRP as well as improve imprinting efficiency. The proposed imprinted biosensor
successfully utilized for detection of HRP with excellent analytical results which linear range is 0.1 mg/mL to 10 ng/mL with LOD of 2.23 ng/mL. Furthermore, electrocatalytical activity of the prepared biosensor toward the reduction of hydrogen
peroxide was investigated in the ranges of 1 to 15 μM with a detection limit of
0.4 μM by using chronoamperometry technique. The developed biosensor was used
for the detection of hydrogen peroxide in unprocessed human plasma sample.
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