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Title
Performance evaluation of ozonation for removal of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa and genes from hospital wastewater
Type of Research Article
Keywords
ozonation; antibiotic-resistant bacteria; wastewater
Abstract
The performance of ozonation for the removal of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) using Escherichia coli and Pseudomonas aeruginosa carrying ARGs from hospital wastewaters was evaluated in this study. Bacterial inactivation was determined using plate count methods and real time PCR for ARG damage (Sul1, blatem, blactx, blavim and qnrS). The reduction rate of bacterial cells and ARGs was increased by different amounts of transferred ozone dose from 11 to 45 mg/L. The concentration of 108 cfu/ml bacteria was reduced to an acceptable level by ozone treatment after a 5 min contact time, Although the removal rate was much higher for concentrations of 106 cfu/ml and 104 cfu/ml bacteria. Overall, the tendency of gene reduction by ozonation from more to less was 16S rRNA > sul1 > blatem > blactx > qnrS > blavim. Given that plasmid-borne ARGs can potentially be transferred to other bacteria even after the disinfection process, our results can provide important insights into the fate of ARGs during hospital wastewater ozonation.
Researchers Farzaneh Baghal Asghari (First Researcher)، Mohammad Hadi Dehghania (Second Researcher)، Reza Dehghanzadeh (Third Researcher)، Davoud Farajzadeh (Fourth Researcher)، (Fifth Researcher)، Amir Hossein Mahvi (Not In First Six Researchers)، Kamyar Yaghmaeian (Not In First Six Researchers)، Akbar Rajabi (Not In First Six Researchers)