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Keywords
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Salinity, probiotic bacteria, Serratia odorifera, wheat, halotolerant
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Abstract
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Soil salinity is one of the most important limiting reasons of crop production in arid and semi arid regions of
Iran. The aim of this study was to investigate the possible amelioration of salt tolerance in wheat crop by
using plant probiotic bacteria. In late spring and early summer of years 2018, healthy plants from
Gramineae family were collected from saline or moderately saline fields of Azarbaijan Shahid Madani
University (37.814589, 45.929741). Roots with surrounded soil was removed from ground and transported
to the laboratory in sterile plastic bags. Halotolerant rhizobacteria were isolated on Nutrient Agar plates
supplemented with 3% NaCl and purified by standard methods. Resulted isolates were screened based on
their effect on wheat seed germination under salt stress. Germination of seeds treated with 12 and 18 dS/m
salinity was drastically affected even in bacterized seeds. Germination of treated seeds with strain R7 under
3 and 6 dS/m salinity was significantly higher than those of other treatments. The effect of this bacterium
on yield and morphophysiologic properties of wheat under salt stress was investigated in hydroponic system
in greenhouse condition. Results showed that treated plants with this strain have higher level of chlorophyll
b, proline, soluble sugars and leaf relative water content. Moreover, the height, dry mass of plants, number
of seed per plant and yield per plant was higher in plants treated with this bacterium in comparison to control
plants. Based on molecular and biochemical characterization this strain was identified as Serratia odorifera.
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