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Abstract
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Tanacetum parthenium L. Schultz-Bip. is an important medicinal plant with valuable secondary
metabolites such as parthenolide. Due to the low amount of active parthenolide in plant tissues, the
establishment of cell and plant lines with larger amounts of this metabolite through genetic engineering is
essential for cost-effective production at the commercial level. Having a reliable tissue culture and
regeneration protocol is critical to the success of plant genetic engineering. In this study, we report reliable
in vitro shoot regeneration protocols for T. parthenium from leaf and internode explants. Using MS as the
basal medium, among the 16 hormonal combinations tested, the highest regeneration rate from leaf
fragments (more than 98%) was obtained on medium containing 0.2 mg L-1 BAP cytokinin and 0.02 mg L-1
NAA auxin. Also, internodes showed the highest percentage of regeneration (85%) in auxin-free medium
containing 0.2 mg L-1 BAP. For both explants, the highest number of regenerated shootlets per explant was
observed in the same media as above. Regenerated plants efficiently performed root formation and
acclimatization to greenhouse condition. Transient expression of GUS gene in leaf explants via gene gun
method proved their suitability for use in gene transfer experiments for molecular breeding programs.
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