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Keywords
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Disinfection, Seed germination, In vitro culture, Medicinal plant, Ammi visnaga
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Abstract
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Increasing population and increasing human tendency to medicinal plants have been among the reasons for researchers' attention to in vitro cultivation of medicinal plants. The science of biotechnology provides the opportunity for micropropagation methods to produce a number of species of medicinal plants by culturing cells, tissues, or organs under in vitro conditions. The Khella belongs to the genus Ammi, an annual or biennial plant native to the Mediterranean region of North Africa, Asia and Europe. This plant has long been used in traditional medicine. Today, it is used in modern medicine to treat many diseases such as colic and coronary artery insufficiency and is used as an antioxidant, antifungal and antibacterial with a larvicidal effect on mosquito larvae. Seed disinfection and germination is one of the critical steps in establishing sterile plant cultures in vitro. Failure to optimize these conditions can lead to lack of optimal removal of contaminants, inefficient germination or the production of abnormal seedlings. In this study, the effect of different disinfection methods on disinfection and seed germination in in vitro cultivation was investigated in a completely randomized and factorial design. The best conditions for seed disinfection were the use of 8% sodium hypochlorite for 15 minutes, and then 70% ethanol for 1 minute. Seed germination percentage was 90% under these conditions. The seedlings produced under these conditions were completely natural.
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