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Abstract
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One of the basic methods for determination of chemical compounds is colorimetry. Use of digital factors like RGB, Hue, HSB etc. as analytical signals by smartphone camera is a good way for using smartphones as alternative for spectrophotometers [1]. Smartphones as
detector convert color values to electrical current signal using CCD or CMOS sensors. Light is received by pixel panels containing electron which absorbs the light and change it to electrical current [2]. Color of metallic nanoparticles is size dependent due to the surface plasmon resonance [3]. Surface Plasmon resonance is the most outstanding optical property of metallic nanostructures. It consists of a collective oscillation of conduction electrons excited by the electromagnetic field of light. In this research we designed a method for determination of silver nanoparticles size based on colorimetry using smartphones. Size controlled synthesis of silver nanoparticles was performed by Shekher Agnihotri and coworkers [4]. Cell holder and imaging box was designed by solid work software and made by FDM 3D printer using poly lactic acid (PLA). Stable conditions for imaging and determination of digital factors needs imaging box and cell holder. Programing codes was used as comparing factors between calibration images color density and unknown sample images. Proposed program selected stable and central point of the sample and compared recorded values with program library.
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