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Abstract
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The Current study was aimed to evaluate the efficacy of hyaluronic acid/Gelatin
(HA/GEL) encapsulated with metformin-loaded mesoporous silica nanoparticles
(MET@MSNs) on the prolonged in vitro proliferation of bone marrow derived human
mesenchymal stem cells (BMhMSCs) without prompting aging [1]. For this propose,
composite scaffolds consisting of MET and MET@MSNs in combination with HA were
fabricated by electrospinning technique [2]. The morphology and biochemical configuration
of fabricated scaffolds were characterized using FESEM, TEM, TGA, and FTIR. The
enhanced proliferation rate and metabolic activity of the BMhMSCs seeded on MET@MSNs
incorporated into HA/Gel scaffolds (MET@MSNs HA/Gel NFs) were confirmed via
PicoGreen and MTT test assay, respectively, over 21 days of culture. The obtained data from
MTT and PicoGreen assays also revealed improved metabolic activity and expansion rate for
BMhMSCs grown on the MET@MSNs-NFs compared to other types of NFs after 14 and 21
days of incubation These results confirmed the capability of MET@MSNs HA/Gel NFs for
persistent and controlled discharge of MET in the design of a novel platform for neutralizing
cellular aging and attaining adequate quantities of fresh BM-hMSCs for tissue engineering
applications.text.
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