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Abstract
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Safe and healthy nutrition has a beneficial effect on human well-being. Various foods, such as
berries, are known to inhibit cancer-promoting pre-proliferative signals. Among fruit and berry crops,
raspberries demonstrate one with the widest ranges of biologically active substances [1]. Ultrasonicassisted
extraction
was
employed
for
highly
efficient
separation
of
secondary
metabolites
from
medicinal
plants.
Specifically,
this
extraction
method
was
able
to
provide
a
higher
content
of
beneficial
compounds
compared
to
conventional
methods
such
as
hot
water
bath,
maceration,
and
soxhlet
extraction,
which
have
been
used
for
many
decades
[2,
3].
The
present
study
aimed
to
the
optimizing
of
black
raspberry
extract
using
ultrasonic-assisted extraction method. Some of main parameters such as the kind of solvent,
extraction time, power and cycle of ultrasonic, and plant to solvent ratio were optimized.
The obtained
results indicated that the optimized conditions were as follows: kind of solvent: methanol 80%; extraction
time: 5 minute; ultrasonic power: 80%; ultrasonic cycle: 6; and plant to solvent ratio: 1:20 w/v. At optimum
condition, the yield of extract was 128/1.043 mg/g dry weight (mg/g DW), whereas the yield of extract with maceration method was 21/1.006
mg/g DW. Also, total phenol and flavonoid contents as well as antioxidant activity of the extracts were
studied. The analysis of total phenol and flavonoid contents was done using the Folin Ciocalteu and
aluminum chloride methods, respectively. Also, DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free
radical was used to test the antioxidant property of the extracts. According to the results, the yield and biological activities of the extract was better than the extract obtained by classical method. So, using this
method, the efficiency and biological activities of the extract were higher and can be used in food and
pharmaceutical industries.
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