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Abstract
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The traditional medicinal practice has been known for centuries in many parts of the
world for the treatment of various human ailments. The use of antibiotics has revolutionized
the treatment of various bacterial infections. However, their indiscriminate use has led to an
alarming increase in antibiotic resistance among microorganisms [1], thus necessitating the
need for the development of novel antimicrobials [2].On the other hand, Foodborne disease is
a global issue with a significant impact on human health. With the growing consumer demand
for natural preservatives to replace chemical compounds, plant antimicrobial compounds must
be thoroughly investigated for their potential to serve as biopreservatives [3]. Therefore, this
study focused on the search for antibacterial agents among natural products. The ethanolic
extracts of 3 plant species of the Apiaceae family, including Pimpinella anisum, Carum
copticum, and Heracleum persicum were evaluated for antibacterial activity using the agar
disk diffusion method against two gram-positive (Bacillus cereus ATCC11778 and Bacillus
subtilis ATCC6633) and two gram-negative (Escherichia coli ATCC25922 and Pseudomonas
aeruginosa ATCC27853) bacteria. The extracts were extracted from dry seeds of the used
plants by ultrasound-assisted extraction method (UAE). All the extracts had an inhibitory
effect against studied bacteria. The most sensitive strain was E. coli, while the most resistant
strain was B. cereus. The highest inhibitory zone was shown by the extract of C. copticum
against E. coli (22.5 mm). Also, the extract of C. copticum was found to be better effective
compared to positive control. Laboratory screening of plant extracts showed encouraging
results indicating their potential uses in the control of selected pathogenic bacteria. According
to the results, C. copticum has the highest antibacterial potential and can be used by the
pharmaceutical, food, and cosmetic industries.
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