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Keywords
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Polyvinylidene fluoride (PVDF), High-throughput, Liquid phase thin film
microextraction, Estrone (E1), 17β-estradiol (E2)
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Abstract
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In this study, n-octanol loaded polyvinylidene fluoride (PVDF) membrane sheet was
introduced as extracting phase in high-throughput liquid phase thin film microextraction
(TFME) for the rapid and simultaneous extraction of two steroid hormones, including estrone
(E1) and 17β-estradiol (E2) in aqueous samples (water, waste water and urine samples) prior
to liquid chromatographic determination. The proposed thin film membrane was characterized
by FE-SEM, AFM, ATR and BET. Thin film membrane was prepared by non-solvent induced
phase inversion method and pore generation using Polyvinylpyrrolidone (PVP). Optimum
extraction/desorption conditions were found as: organic solvent: n-Octanol, Salt
concentration: 16%w/v NaCl, stirring speed: 1600 rpm, pH 8, extraction temperature: 25 °C,
extraction time: 35 min, desorption solvent: methanol and desorption time: 15 min. The
figures of merit were obtained under the optimized conditions: the Linear Dynamic Ranges
(LDRs): 6.70-100 μg L-1 and 4.18-100 μg L-1, Limits of Detection (LODs): 2.21 μg L-1 and
1.38 μg L-1, Limits of Quantification (LOQs): 6.70 μg L-1 and 4.18 μg L-1 for E1 and E2,
respectively.
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