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Abstract
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Side effects caused by bone fractures and restrictions on bone
regeneration impose an enormous economic burden on the
health system of society. To overcome these limitations, tissue
engineering and cell-based therapies have been proposed as
alternatives to induce and promote bone healing. Still, bone
regeneration disadvantages, such as limited and painful surgery,
the risk of infection, nerve injury, bleeding, and function damage,
have led investigators to find an alternative therapy. In some
studies, bone stimulants have prompted scientists to design scaffolds with appropriate physical structure with the possibility of
cell adhesion and proliferation, which plays an influential role in
the regeneration and repair of bone tissue. PCL nanofiber is an
absorbing candidate for the formulation of biocompatible scaffolds used in tissue engineering. To overcome these negative
aspects, improve the properties of PCL nanofibers, and based on
the biocompatibility and superior mechanical properties of POSS,
Polyhedral Oligomeric Silsesquioxane-Polycaprolactone-Zeolite
(POSS-PCL-Zeolite) nanocomposite electrospun nanofiber scaffolds
were fabricated in the present study. Nanohybrids and nanofibers
structures were characterized by FTIR, HNMR, XRD, SEM, EDX, and
DSC techniques. We used cellular and molecular assays, including
DCFH ROS detection system, gene expression (RUNX-2,
Osteocalcin, Nrf2, BAX, VEGF gens), and apoptotic to demonstrate
the biocompatibility and induce bone differentiation of formulated POSS-PCL-Zeolite scaffolds. The results showed the biodegradability of POSS-PCL-Zeolite Nano-scaffold and supported
the nesting of mesenchymal stem cells (MSCs) and induced bone
differentiation by POSS-PCL-Zeolite Nano-scaffold
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