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Abstract
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A magnetic Mg/Fe layered double hydroxide (magnetic LDH) nanocomposite was successfully pre-
pared and used as a suitable adsorbent to simultaneously extract vitamins B1 and B6 from com-
plex infant formula and food supplements. The important parameters affecting the extraction
were optimized and the final parameters are as follows; the amount of adsorbent (20 mg), extrac-
tion time (6 min), elution time (8 min), and elution solvent ethanol in 750 mL. As-prepared nanoad-
sorbent was successfully in magnetic solid phase extraction set-up with no hazardous solvents
and extraction performed in 6 min. The structure of nanoadsorbnet is lamellar, providing a wide
surface to extract the analytes. The quantification of the analytes was accomplished using the
liquid chromatography-tandem mass (LC/MS-MS) technique. Under optimum extraction conditions,
the linearity ranged from 4 to 1000 ng/mL (vitamin B1) and 20 to 1000 ng/mL (vitamin B6), and
the correlation of coefficients (R2
) was obtained better than 0.99. The intra-day (n 1⁄4 3) and inter-
day precisions (n 1⁄4 3 working days) calculated in the form of percent relative standard deviations
(%RSDs) were obtained below 10.20%. The proposed method was successfully practiced for ana-
lyzing vitamins B1 and B6 in several brands of infant formulas and food supplements.
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