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Abstract
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An electrochemical immunosensing platform was developed for the detection of receptor tyrosine kinase-orphan receptor-
2 (ROR2) at a glassy carbon electrode (GCE) modified with the electrospun nanofiber containing polyvinylpyrrolidone
(PVP), soy, and Au nanoparticles (AuNPs). The PVP/soy/AuNP nanofiber exhibited good electrochemical behavior due to
synergistic effects between PVP, soy, and AuNPs. The PVP/soy in the modified film provided good mechanical strength,
high porosity, flexible structures, and high specific surface area. On the other hand, the presence of AuNPs effectively
improved conductivity, as well as the immobilization of anti-ROR2 on the modified GCE, leading to enhanced sensitivity.
Various characterization approaches such as FE-SEM, FTIR, and EDS were used for investigating the morphological and
structural features, and the elemental composition. The designed immunosensor performance was investigated using electrochemical
impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). Under
optimum conditions with a working potential range from −0.2 to 0.6 V (vs. SCE), sensitivity, linear range (LR), limit of
detection (LOD), and correlation coefficient (R2) were acquired at 122.26 μA/cm2 dec, 0.01–1000 pg/mL, 3.39 fg/mL, and
0.9974, respectively. Furthermore, the determination of ROR2 in human plasma samples using the designed immunosensing
platform was examined and exhibited satisfactory results including good selectivity against other proteins, reproducibility,
and cyclic stability.
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