Keywords
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biocompatibility, biomedical applications, electrospinning, mechanical properties, polyesters
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Abstract
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In this study, the osteo-inductive potential of Prunus amygdalus dulcis
(SA) extract that loaded with different concentrations in poly-ε-Caprolactone/
gelatin (PCL/GEL) nanofibrous scaffolds was investigated. The morphology
and composition of nanofibers were evaluated using field emission scanning
electron microscope and FT-IR spectroscopy. Mechanical properties, hydrophilicity
and pore structures were determined by tensile test, contact angle, and
N2 adsorption–desorption isotherm, respectively. By incorporation of SA
extract into PCL/GEL nanofibers, mechanical capabilities as well as their physical
qualities were improved. The response of human dental pulp stem cells on
prepared scaffolds were studied using MTT assay, alkaline phosphatase activity,
alizarin red assay, and quantitave real-time PCR analysis. The SA loaded
nanofibers presented excellent cell attachment and enhanced osteoblast proliferation
and mineralization compared to the pristine PCL/GEL nanofibers. The
analysis revealed the addition SA extract to PCL/GEL nanofibers increased the
expression of osteogenic genes significantly. According to our findings,
PCL/GEL/SA nanofibers show a lot of potential for bone tissue engineering.
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