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Abstract
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The hallmarks of malignant illnesses include defective DNA repair capability and disruption of genomic integrity brought on by the accumulation of different types of DNA damage. In the case of breast cancer, previous research has suggested that specific SNPs within genes involved in DNA repair and tumor suppression pathways, such as OGG1 and TP53, may modulate an individual’s susceptibility to the disease.TP53 and OGG1 play a significant role in genome integrity and DNA repair. The genetic variation in TP53 and OGG1 reduces the repair function and increases cancer risk. The present study aimed to evaluate the single nucleotide polymorphisms of these two genes to show the combined effect of TP53 and OGG1 genotypes in breast cancer. The present case-control study was carried out by collecting blood samples from 166 patients with breast cancer and 166 individuals without a personal history of breast cancer. Tetra-ARMS and AS-PCR were used to ascertain the genotypes. The online Javastat statistics package, MDR, and IBM SPSS Statistics 26 were used to analyze the data. In silico analysis was performed to evaluate the effects of OGG1 and TP53. A strong synergistic interaction between TP53 and OGG1, moderate synergistic interaction between OGG1 and age, and correlation between TP53 and age was indicated, suggesting a novel interaction between TP53 and OGG1. Moreover, the recessive model of the OGG1 Ser326Cys polymorphism (P = 0.039) had a protective effect against breast tumors. We found no significant relationship between TP53 Arg72Pro and susceptibility to breast cancer. In this study, the results suggested the OGG1- Ser326Cys GG genotype as a protective factor against the risk of breast cancer in a representative sample of Iranian-Azeri women. Also, using the MDR method, we discovered a gene-gene association between TP53 and OGG1.
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