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Abstract
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Stachys schtschegleevii is an important medicinal plant, containing highly valuable compounds such as verbascoside, pinene, limonene, and myrcene. In this study, we report efficient in vitro callus induction and shoot regeneration protocols for S. stchschegleevii from two different explant sources of leaf and internode. The highest callus induction rate was observed in
internode explants using MS medium complemented with 0.3 mg/l TDZ and 0.7 mg/l 2,4-D. The resulting calli were embryogenic with the ability to grow and maintain their structure on MS medium containing 1.5 mg/l BAP at high efficiencies (90%). The β-gulucoronidase (gus) gene was then successfully transferred to the internode explants by the particle gun method, demonstrating their potential to express foreign genes. In conclusion, here we report efficient protocols for callus induction and regeneration of S. schtschegleevii that can be used in germplasm maintenance, production of secondary metabolites in in vitro cultures, and molecular breeding through genetic engineering.
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