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Abstract
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Abstract: Marine microalgae have been used as a source of nutritional and medicinal supplements for many years due to the presence of various compounds and nutrients. Bioactive compounds with algal origin have many capabilities such as antihypertensive, immune modulator, antioxidant, anticancer and antimicrobial. Proteins make up a high percentage of compounds in microalgae. Bioactive peptides are compounds that are formed after enzymatic hydrolysis of proteins and have different properties and functions. This study investigated in-silico the properties of bioactive peptides in the phycoerythrin protein of Porphyridium purpureum microalgae. Phycoerythrin protein, which is one of the effective proteins in the photosynthesis of microalgae, has two subunits, alpha and beta. The bioinformatics investigation of potential peptides in these subunits in 4 selected fields with activities related to human health, determined that the frequency of occurrence of these peptides in the alpha subunit is higher than beta. Most of these peptides have DPP-4 inhibitory properties and then ACE inhibitory properties, which are effective in improving type 2 diabetes and high blood pressure, respectively. Also, by simulating the effect of digestive enzymes (pepsin, trypsin, and chymotrypsin) on phycoerythrin protein subunits, 15 (non-toxic) peptide fragments with different biological activities were created, and the abundance, activity potential, and digestive absorption of these peptides were also investigated. The results of this study can be a basis for further experimental and laboratory investigations to make medicine and food supplements from this protein and its peptides.
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