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Abstract
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This research presents a selective and sensitive electrochemical biosensor for the detection of the mesenchymal–
epithelial transition factor (c-MET). The biosensing is based on a modification of the SPCE (screenprinted
carbon electrode) with the electrospun nanofiber containing eudragit (EU), hydroxypropyl methylcellulose
(HPMC), and Zeolite imidazolate frameworks (ZIF-8) nanoparticles. EU/HPMC/ZIF-8 nanofibers have
presented a high capability of electron transfer, and more active surface area than bare SPCE due to synergistic
effects between EU, HPMC, and ZIF-8. On the other hand, EU/HPMC nanofibers provided high porosity, flexible
structures, high specific surface area, and good mechanical strength. The presence of ZIF-8 nanoparticles
improved the immobilization of anti-c-MET on the modified SPCE and also resulted in increasing the conductivity.
By c-MET incubation on the modified SPCE, c-MET was connected to anti-c-MET, and consequently the
electrochemical signal of [Fe(CN)6]3-/4- as the anion redox probe was reduced. In order to investigate the
structural and morphological characteristics and elemental composition of electrospun nanofibers, various
characterization methods including FE-SEM, XRD, FTIR, and EDS were used. Under optimum conditions with a
working potential range 0.3 0.6 V (vs. Ag/AgCl), linear range (LR), correlation coefficient (R2), sensitivity,
and limit of detection (LOD) were acquired at 100 fg/mL-100 ng/mL, 0.9985, 53.28 μA/cm2.dec, and 1.28 fg/
mL, respectively. Moreover, the mentioned biosensor was investigated in a human plasma sample to determine c-
MET and showed ideal results including reproducibility, stability, and good selectivity against other proteins.
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