Research Specifications

Title	Extraction, Purification and Characterization of Immunomodulatory Effects of Cyclotides Isolated from Viola Odorata
Type of Research	Article
Keywords	Plant cyclic peptides have displayed a diverse range of biological activities. Cyclic peptides exist in different plant families, such as Violaceae.
Abstract	In this study, the existence of cyclic peptides in Viola odorata has been investigated. The plant materials were subjected to maceration in methanol: CH2-Cl2 (1:1; v/v), and then the crude extract passed through the C18 column by vacuum liquid chromatography method and fractionated into 50% and 80 % ethanol extract. The obtained fractions were analyzed by HPLC and MALDI-TOF. Finally, the 50% and 80% fractions were injected into the female C57BL/6 mice intraperitoneally at doses of 5, 50, and 100 mg/kg. The lymphocyte, monocyte, neutrophil, eosinophil, and white blood cell (WBC) counts of whole blood in different groups were compared before and 24 hours after the injection. The results confirmed the presence of cyclic peptides in Viola odorata
Researchers	Ladan Dayani (First Researcher)، Azadeh Taheri (Second Researcher)، Jaleh Varshosaz (Third Researcher)، Mehdi Aliomrani (Fourth Researcher)، Masoud Sadeghi Dinani (Fifth Researcher)، Hossein Hashempour (Not In First Six Researchers)