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Abstract
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Poppy with the scientific name Papaver somniferum L. belongs to the Papaveraceae family. More than 40 types of alkaloids
are known in poppy, the most important of which are morphine, codeine, thebaine, noscapine and papaverine(Hayat,
Hameed, & Zia-Ul-Haq, 2023). Alkaloids of the morphine group in poppy are synthesized from two amino acid L-tyrosine
molecules through 17 enzymatic steps during several continuous oxidative reactions(Aghaali & Naghavi, 2024). The first
key enzyme in this pathway is L-tyrosine decarboxylase (TYDC2). In this research, TYDC2 gene was compared and
investigated in four tissues of poppy plant. The sequence of these tissues (root, leaf, capsule and petal) was identified by
NGS method and studied bioinformatically, these sequences were selected from the Gene section of NCBI website and
downloaded by SRAtoolkit tool and their quality was determined by the tool. fastqc was evaluated and gene expression
graphs and heatmap graphs were drawn by Rstudio. Finally, it was analyzed and evaluated by gprofiler in terms of gene
ontology. In this research, the expression level of the production gene was different in the mentioned tissues and the
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4th International Iranian Conference on
Bioinformatic
Zanjan
February 4-6. 2025
Paper Num: ICB13.2025…….
4th International Iranian Conference on
Bioinformatic
Zanjan
February 4-6. 2025expression level was detected in the leaf tissue more than the other tested tissues, and the lowest expression level was
observed in the root tissue. Finally, gene ontology, genes were also examined and it was found that; The molecular function
and the extent of the biological process of this gene play a great role in the catalytic activity. It has been observed in various
plant species that the biosynthesis of a large number of primary metabolites as well as secondary metabolites are dependent
on this gene. Gene expression is a process in which the information contained in a gene is used to produce a f
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