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Abstract
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Skin aging is a complex process, influenced by internal and external factors, especially the sun's UV, which can lead to premature aging by damaging collagen and other skin structures. Today, protecting the skin using compounds with biological sources is so important. Exosomes are naturally small membrane vesicles (30-150 nm) of endocytosis origin and have unique properties, including low immunogenicity, biodegradability, and low-level toxicity, which have been increasingly noticed. According to this issue, the microalga Dunalliela salina was selected for the preparation of exosomal nanovesicles and human skin fibroblast treatment in this research. Exosomes derived from D. salina have antioxidant and anti-inflammatory properties. The extraction process led to exosomes with dimensions of 57.58 to 76.08 nm. The size of exosomes was measured using a Dynamic light scattering device. The results obtained from the simultaneous cultivation of exosomes and skin cells in DMEM medium with high glucose and 10 to 20% FBF in a 37°C incubator for 24 hours led to a 20% increase in the proliferation and rejuvenation of skin cells. The results indicate that these exosomes have significant potential as anti-aging agents. According to these findings, microalgae-derived exosomes can lead to the design and development of new solutions in skin care.
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