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Abstract
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Abstract Association of P53 polymorphisms with the increased
risk of various cancers has been investigated in numerous
studies. However, the results were conflicting and no
polymorphism has been determined as a definite risk factor. It
is likely that the study of P53 combined genotypes and haplotypes
may be more useful than individual polymorphisms.
Thus, in this study, we analyzed the associations of intron 3
Ins16bp and exon 4 Arg72Pro polymorphisms, aswell as their
combined genotypes and haplotypes with the risk of differentiated
thyroid carcinoma in Iranian-Azeri patients. This case–
control study was performed on 84 Iranian Azeri patients with
differentiated thyroid carcinoma and 150 healthy subjects.
Intron 3 genotype was determined using PCR products analysis
on polyacrylamide gels and AS-PCR was used for
genotyping Arg72Pro polymorphism. The javastat online statistics
package software and SHEsis program were applied for
data analysis. There was no significant difference in genotype
frequencies of both two polymorphisms between cases and
controls. However, the (−16ins/−16ins) (Arg/Pro) genotype
combination had a noticeable but not significant association
with decreased risk of thyroid cancer development (OR=
0.497 95%CI: 0.209–1.168 P=0.080) and also the frequency
of (−16ins-Pro) haplotype was significantly higher in controls
rather than patients (OR=0.543 95%CI: 0.326–0.903
P=0.018). In our study, there was association between
(−16ins-Pro) haplotype with decreased risk of differentiated
thyroid carcinoma development in Iranian-Azeri patients.
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