مشخصات پژوهش

The potato tuber moth, Phthorimaea operculella is a highly destructive pest of potato crops, causing significant losses in both field and storage conditions. Due to the environmental and health risks posed by synthetic pes- ticides, as well as increasing insecticide resistance, sustainable alternatives for integrated pest management (IPM) are urgently needed. This study investigates the synergistic potential of Achillea millefolium (yarrow) ex- tracts and three entomopathogenic nematodes (EPN) species including Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora against P. operculella larvae. Bioassays were conducted on first-instar (3-day-old) larvae reared on potato tubers under controlled conditions. Yarrow aerial parts were extracted via maceration using hexane, ethyl acetate, methanol, and water solvents. The insecticidal effects of individual and combined treatments were assessed, with LC50 values calculated for each. Key findings revealed that the methanol extract exhibited the highest larvicidal activity (LC50 = 9.030 g/L), while the ethyl acetate extract was least effective (LC50 = 24.898 g/L). Among EPNs, S. carpocapsae was the most virulent (LC50 = 34.88 IJ/100 μL), whereas H. bacteriophora showed the lowest efficacy (LC50 = 114.3 IJ/100 μL). Notably, the methanol extract negatively impacted all three EPN species, exhibiting complete antagonism in combined treatments. In contrast, ethyl ac- etate extract demonstrated the strongest synergistic effect with EPNs. Greenhouse trials, conducted at 10 × LC50 concentrations, confirmed that combined treatments significantly reduced larval mining damage compared to individual applications. These findings highlight the potential of A. millefolium-EPN combinations as a sustain- able IPM strategy against P. operculella, with solvent selection critically influencing compatibility.