مشخصات پژوهش

Microfluidic chips are coin biotechnology research1. Microfluidic chip technology offers a promising, lowcost, and easy-to-implement platform for developing efficient seperation methodologies2. This study presents a separation method based on two different microfluidic chip designs. As compact microfluidic designs offer advantages in speed, efficiency and cost3 . Implementation of three inlets with usage of an applied electric current enables a separation based on molecular charge or mobility, allowing for precise and active control during the process. On this current based chip middle inlet introduces plant extract, with other inlets adding reagents. To achive the optimum conditions for seperation different voltages are utilized. The other chip features two inlets and contains a PET membrane placed between two separate channels, enabling passive separation through selective diffusion without the need for external forces. The membranebased design offers a simple and cost-effective alternative, suited for small-scale laboratory applications. In membrane-based design dilute acid solution either HCl or phosphoric acid was introduced into the second inlet. Flow rates of 0.5 L/min, 1 L/min, and 2 L/min were used during testing. To evaluate the method s effectiveness, a plant extract from Viola ignobilis, collected in Iran s West Azerbaijan province used in both design applications. Cyclotides are the target compounds in the plant extract. They are small, highly stable cyclic peptides of interest for their potential biomedical applications4.