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Abstract
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most important medicinal herbs with diverse
medical and industrial applications. In the present
study, genetic diversity and relationships of 12 Iranian
and two exotic populations of Melissa officinalis L.
were analyzed using inter-retrotransposon amplified
polymorphism (IRAP) and retrotransposon-microsatellite
amplified polymorphism (REMAP) markers.
Both markers’ systems revealed a high level of polymorphism
and the average percent polymorphism for
IRAP and REMAP was 96.35% and 90.78%, respectively.
The IRAP with mean polymorphic information
content (PIC) of 0.27 and marker index (MI) of
14.4 was more polymorphic compared with REMAP
(PIC = 0.28, MI = 11.7). Distance- and model-based
cluster analyses and principal coordinate analysis
results showed that the populations from Germany
and Japan were closely clustered. The individuals
from Iranian populations in most cases were also
grouped together based on their origins. Our study
revealed a high level of insertional polymorphism in
lemon balm populations based on primers designed
using barley’s retrotransposons families.
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