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Abstract
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In this study, a novel dispersive magnetic solid-phase extraction (DMSPE) method utilizing a magnetite/clinoptilolite
zeolite nanocomposite was developed for the extraction and pre-concentration of triptorelin acetate (TA) from aqueous
solutions. The pre-concentrated analyte was quantified using high-performance liquid chromatography with ultraviolet
detection (HPLC-UV). The magnetic nanocomposite was synthesized and characterized for its structural, morphological,
and magnetic properties using field emission scanning electron microscopy (FESEM), powder X-ray diffraction (XRD),
Fourier transform infrared spectroscopy (FTIR), nitrogen adsorption–desorption isotherms (BET), and vibrating sample
magnetometry (VSM). Key parameters affecting extraction efficiency including pH, temperature, extraction and desorption
times, sorbent amount, and the type, volume, and concentration of the eluent were systematically investigated and
optimized. Method validation was performed under optimal conditions. The calibration curve for TA was linear over the
range of 5.0–250.0 ng mL‒1 with a correlation coefficient of 0.9988. The DMSPE–HPLC-UV method achieved a detection
limit of 1.1 ng mL‒1 and a pre-concentration factor of 100. Its applicability was demonstrated by successfully detecting
TA in various urine samples.
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