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Abstract
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A drug delivery system (DDS) is defined as a formulation or a device that enables the
intruduction of a therapeutic substance in the body and improves its efficacy and safety [1].
One of the main goals of scientists in nanomedicine for cancer therapy is expansion of a smart
carrier that can increase the penetrance and survival of drugs in tumors and decrease the side
effect and toxicity related with most anticancer drugs [2]. Dual anticancer drug delivery
systems can enhance the therapeutic effect for cancer therapy [3]. In this study a novel
antibacterial nanocarrier was syntesized. The synthesis process and prepared polymeric nanosystem
were characterized and validated by FTIR, 1HNMR, SEM and TGA methods. SEM
results revealed that nanoparticles presented a homogenous morphology with mean diameter
of around 100 nm. Its bactericidal activity was evaluated by determining minimum inhibitory
concentration (MIC) values against several of bacterias and funguses. Then, two different
anticancer drugs, doxorubicin (DOX) and methotrexate (MTX) were conjugated
simultaneously to nanocarrier by hydrogen bonding and ionic interaction. Encapsulation
efficiency was about 95% for both drugs. MTX–DOX conjugated nanocarrier yielded a pH
and temperature-triggered release of entrapped drugs at tumor tissue environment (lower pH
and higher temperature than physiological condition). Cell viability experiments confirmed
that the co-administration of DOX with MTX caused an efficient anticancer performance to
MCF7 cell lines verified by qRT-PCR and MTT assay tests. The results indicated that this
carrier is one of the most effective systems for certain treat of cancer patients.
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