مشخصات پژوهش

A drug delivery system (DDS) is defined as a formulation or a device that enables the intruduction of a therapeutic substance in the body and improves its efficacy and safety [1]. One of the main goals of scientists in nanomedicine for cancer therapy is expansion of a smart carrier that can increase the penetrance and survival of drugs in tumors and decrease the side effect and toxicity related with most anticancer drugs [2]. Dual anticancer drug delivery systems can enhance the therapeutic effect for cancer therapy [3]. In this study a novel antibacterial nanocarrier was syntesized. The synthesis process and prepared polymeric nanosystem were characterized and validated by FTIR, 1HNMR, SEM and TGA methods. SEM results revealed that nanoparticles presented a homogenous morphology with mean diameter of around 100 nm. Its bactericidal activity was evaluated by determining minimum inhibitory concentration (MIC) values against several of bacterias and funguses. Then, two different anticancer drugs, doxorubicin (DOX) and methotrexate (MTX) were conjugated simultaneously to nanocarrier by hydrogen bonding and ionic interaction. Encapsulation efficiency was about 95% for both drugs. MTX–DOX conjugated nanocarrier yielded a pH and temperature-triggered release of entrapped drugs at tumor tissue environment (lower pH and higher temperature than physiological condition). Cell viability experiments confirmed that the co-administration of DOX with MTX caused an efficient anticancer performance to MCF7 cell lines verified by qRT-PCR and MTT assay tests. The results indicated that this carrier is one of the most effective systems for certain treat of cancer patients.