|
Abstract
|
In this study, a practical approach has been developed to detect ovarian specific antigen (CA-125) with high
sensitivity and specificity. The method utilizes enhanced differential pulse voltammogram signals of a selfassembled prussian blue nanoparticles (PB) decorated on MXene Quantum dots (QDs) supported by electrodeposited Au nanoparticles modified glassy carbon electrode (AuNP/PB/MXene QD/GCE). The fabrication
procedure was completed by casting an optimized value of streptavidin and CA-125 antibody on the transducing
platform. The improved electrochemical characteristics of the MXene QD/PB/AuNPs modification were ascribed
to the combined impacts of MXene QD, PB, and AuNPs. The integration of MXene QD-PB composite within the
modified layer contributed to enhanced mechanical strength. Additionally, the inclusion of gold nanoparticles
(AuNPs) notably enhanced conductivity and promoted the attachment of anti-CA-125 onto the modified glassy
carbon electrode (GCE), consequently improving sensitivity. Several analytical methods, such as energydispersive X-ray spectroscopy (EDS) and field emission scanning electron microscopy (FE-SEM) were utilized
to investigate the structural, elemental composition and morphological characteristics. The functionality of the
newly created immunosensor was evaluated using electrochemical impedance spectroscopy, differential pulse
voltammetry and cyclic voltammetry to evaluate the synergistic effects. By employing this highly efficient
analysis strategy, the measurement of CA-125 with differential pulse voltammetry (DPV) results a remarkable
sensitivity with a detection limit of 0.57 pU.mL− 1 and a wide linear range of 1 pU.mL− 1 to 0.12 nU.mL− 1 (R2 =
0.9824). Comparable results were obtained by analyzing serum samples from ovarian cancer patients at various
stages. This study offers a significant insight into the advancement of immunosensor techniques for fabricating
electrochemical transducers for the clinical detection of CA-125.
|